Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析          下载免费PDF全文

武翠玲  宋英达  高慧芳  张廉芸  任晨霞 《盐湖研究》2020,28(1)

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性，属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高，α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高，且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸，并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示，α螺旋和β片层围成了蛋白酶的催化腔，EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体，且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性，并有助于其催化功能的发挥，为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。  相似文献   

Simulating cross-sectional geometry of the main channel in response to changes in water and sediment in Lower Yellow River     

Wang  Yanjun  Wu  Baosheng  Zhong  Deyu 《地理学报(英文版)》2020,30(12):2033-2052

Journal of Geographical Sciences - To understand the non-equilibrium morphological adjustment of a river in response to environmental changes, it is essential to (i) accurately identify how past...  相似文献   

An overview of clustering methods for geo-referenced time series: from one-way clustering to co- and tri-clustering     

Xiaojing Wu  Raul Zurita-Milla  Changqing Song 《International journal of geographical information science》2020,34(9):1822-1848

  相似文献   

A machine learning approach for predicting computational intensity and domain decomposition in parallel geoprocessing     

Peng Yue  Fan Gao  Boyi Shangguan  Zheren Yan 《International journal of geographical information science》2020,34(11):2243-2274

ABSTRACT

High performance computing is required for fast geoprocessing of geospatial big data. Using spatial domains to represent computational intensity (CIT) and domain decomposition for parallelism are prominent strategies when designing parallel geoprocessing applications. Traditional domain decomposition is limited in evaluating the computational intensity, which often results in load imbalance and poor parallel performance. From the data science perspective, machine learning from Artificial Intelligence (AI) shows promise for better CIT evaluation. This paper proposes a machine learning approach for predicting computational intensity, followed by an optimized domain decomposition, which divides the spatial domain into balanced subdivisions based on the predicted CIT to achieve better parallel performance. The approach provides a reference framework on how various machine learning methods including feature selection and model training can be used in predicting computational intensity and optimizing parallel geoprocessing against different cases. Some comparative experiments between the approach and traditional methods were performed using the two cases, DEM generation from point clouds and spatial intersection on vector data. The results not only demonstrate the advantage of the approach, but also provide hints on how traditional GIS computation can be improved by the AI machine learning.  相似文献   

长江经济带开发区空间分布与产业集聚特征研究   总被引：2，自引：2，他引：0  

唐承丽  陈伟杨  吴佳敏  周国华  王美霞  郭夏爽 《地理科学》2020,40(4):657-664

采用最近邻指数、Ripley’s L 函数、核密度估计等方法分析长江经济带开发区的空间分布与产业集聚特征,结果表明:① 长江经济带开发区总体上呈东密西疏、东强西弱、东中西段分异的显著集聚分布特征;② 基于主导产业划分的各类开发区在空间上均为集聚分布,集聚强度和规模随距离的增加基本都呈“先增加后减小”的规律,集聚形态各异,主要有“单核心”“双核心”“多核心”3种;③ 长江经济带东段地区主要以装备制造、通信电子、汽车制造、新材料、生物医药等资本技术密集型产业集聚为主,中、西段地区则集聚了化学工业、金属加工、食品制造、纺织服装等资本与劳动密集型产业。要加强经济带上中下游开发区之间的多维良性互动,注重绿色发展、创新发展与结构优化,进一步提升其对长江经济带高质量发展的引领与支撑作用。  相似文献   

Spatiotemporal analysis of COVID-19 risk in Guangdong Province based on population migration     

Ye  Yuyao  Wang  Changjian  Zhang  Hong’ou  Yang  Ji  Liu  Zhengqian  Wu  Kangmin  Deng  Yingbin 《地理学报(英文版)》2020,30(12):1985-2001

Journal of Geographical Sciences - Population migration, especially population inflow from epidemic areas, is a key source of the risk related to the coronavirus disease 2019 (COVID-19) epidemic....  相似文献   

Building national eco-cultural protection areas and the Xiangxi practice in Hunan Province     

Liu  Chunla  Wu  Liping  Xu  Mei  Zeng  Fanchao  Jiao  Lipeng 《地理学报(英文版)》2020,30(12):2076-2092

Journal of Geographical Sciences - Since 2007, the Chinese government has initiated the building of national eco-cultural protection areas (NECPAs), thereby embarking on a significant...  相似文献   

Risk evaluation of dam-break environmental impacts based on the set pair analysis and cloud model     

Ge  Wei  Li  Zongkun  Li  Wei  Wu  Meimei  Li  Juanjuan  Pan  Yipeng 《Natural Hazards》2020,104(2):1641-1653

Natural Hazards - Many dams have been constructed around the world. Compared with the potential losses of life and economy, the environmental impacts caused by dam breach were less analyzed. As a...  相似文献   

Full moment tensor inversion constrained by double-couple focal mechanism for induced seismicity*          下载免费PDF全文

Yuyang Tan  Haijiang Zhang  Junlun Li  Chen Yin  Furong Wu 《地震科学（英文版）》2020,33(4):177-193

In this study, we propose a new method to determine full moment tensor solution for induced seismicity. This method generalizes the full waveform matching algorithm we have developed to determine the double-couple (DC) focal mechanism based on the neighbourhood algorithm. One major difference between the new method and the former one is that we adopt a new misfit function to constrain the candidate moment tensor solutions with respect to a reference DC solution in addition to other misfit terms characterizing the waveform matching. Through synthetic tests using a real passive seismic survey geometry, the results show the new constraint can help better recover the DC components of inverted moment tensors. We further investigate how errors in the velocity model and source location affect the moment tensor solution. The synthetic test results indicate that the constrained inversion is robust in recovering both the DC and non-DC components. We also test the proposed method on several real induced events in an oil/gas field in Oman using the same observation system as synthetic tests. While it is found that the full moment tensor solutions without using the DC constraints have much larger non-DC components than solutions with the DC constraints, both solutions are able to fit the observed waveforms at similar levels. The synthetic and real test results suggest the proposed DC constrained inversion method can reliably retrieve full moment tensor solutions for the induced seismicity.  相似文献   

曲靖非相干散射雷达电离层F区日间电子温度变化特征初步分析   总被引：1，自引：0，他引：1       下载免费PDF全文

丁宗华  代连东  杨嵩  唐志美  吴健  许正文 《地球物理学报》2020,63(4):1282-1293

利用曲靖非相干散射雷达2017-2018年春夏季观测数据首次分析了电离层日间150~450km电子温度的地方时与高度变化特征及其与电子密度的相关性.发现hmF2及以上的电子温度在日出日落时具有两个峰值,在11∶00-16∶00LT之间变化较小,高度越高午后上升的时间越早;从150km开始迅速增加,在约220km达到最大值,然后开始降低,在约300~350km达到最小值,最后单调上升;200km以下电子温度与电子密度成正相关(主要由热传导控制),200~450km之间存在明显的反相关(光电离过程占主导),电子-离子温度差与电子密度对数之间存在近似线性关系,电子温度逐日变化与光电离因子的变化趋势相似,这种相关性在中午与午后更明显;以上结果与其他非相干散射雷达观测和电离层模型计算结果基本一致,但也存在一些差别,需要结合更多数据深入分析.  相似文献